The Partenopei face a duanting trip to Germany on April 16, with Dieter Hecking’s side having comfortably seen off Inter in the last round.
Fiorentina will also play away from home in the first leg, with the Viola set to make a 4,000km round-trip to Ukraine.
That represents significantly more travelling for Vincenzo Montella’s men, who emerged victorious from an all-Italian tie with Roma in the Last 16.
Elsewhere, the two lowest-ranked sides in the draw were paired with Dnipro drawing Club Brugge.
Last year’s winners Sevilla will take on Zenit St Petersburg, who saw off Torino in the previous round.
The return legs will all take place on April 23.
Europa League Quarter-Finals Second Leg
Zenit v Sevilla
Club Brugge v Dnipro – Buy Europa League Tickets
Fiorentina v Dynamo Kiev – Buy Europa League Tickets
Napoli v Wolfsburg – Buy Europa League Tickets